400m Women 1st round, Heat:2 of 2 ; Official result since 17:33
| Rank | Lane | ID | Name | SB | Team | Cat | Result | Info | Video |
| 1 | 5 | 82 | Rita OSSAI | NGR | Jun | 53.97 | Q | ||
| 2 | 2 | 107 | Halima NAKAAYI | UGA | Jun | 55.07 | Q | ||
| 3 | 6 | 25 | Mahlet MULUGETA | ETH | Jun | 55.94 | Q | ||
| 4 | 8 | 96 | Janet SEELIGER | RSA | Jun | 57.57 | q | ||
| 5 | 1 | 10 | Galefele MOROKO | BOT | Jun | 57.73 | |||
| 6 | 3 | 92 | Carina SWIEGERS | RSA | Jun | 59.25 | |||
| 7 | 7 | 104 | Nafy MANE | SEN | Jun | 59.74 | |||
| 4 | 65 | Aurelie ALCINDOR | MRI | Jun | DNF |
