| Rank | Lane | ID | Name | PB | Team | Cat | Result | Info | Video |
| 1 | 6 | 506 | FORTE Julian | 10.03 | JAM | 10.11 | Q | ||
| 2 | 4 | 504 | CARTER Nesta | 9.78 | JAM | 10.13 | Q | ||
| 3 | 5 | 505 | SIMBINE Akani | 9.97 | RSA | 10.21 | Q | ||
| 4 | 7 | 507 | PEREZ Yunier | 10.22 | TUR | 10.32 | q | ||
| 5 | 2 | 502 | PAULINA Hensley | 10.26 | NED | 10.32 | q | ||
| 6 | 8 | 508 | VAN LUIJK Patrick | 10.25 | NED | 10.54 | |||
| 7 | 3 | 503 | AIKINES-ARYEETEY Harry | 10.08 | GBR | 10.63 | |||
| 8 | 9 | 509 | MEYER Gaeonn | 10.82 | NED | 10.89 | |||
| 9 | 1 | 501 | LIONAHR Serencio | 11.07 | NED | 11.02 |